How to re-cluster PFGE restriction profiles from gel pictures noting that i do not have access to bionumerics?

We have the results of pulsed field gel electrophoresis PFGE of some bacterial strains , and we clustered bacterial strains with bionumerics in fact, we are no more able to use the software since...

31 December 2018 409 0 View

S1 PFGE for visualizing megaplasmids, what differs from a ususal PFGE (other than using S1 instead of res enzyme)?

The question is about S1 PFGE ; a pulsed field in which S1 is used to linearize plasmidic DNA so that the latter could migrate through pulsed elecrophoresis. i intend to run a usual PFGE, i wanted...

02 March 2018 1,037 2 View

I would like to know the impact factor of 'Journal of Chemical Biology' ??

09 October 2015 5,319 1 View

Why use mineral oil in RAPD experiments and which one?

It is indicated that RAPD reactions are made with a layer of mineral oil on the top of the mixture, iI would like to ask which oil to use and how and why it is used? I actualy did the experiment,...

09 October 2014 2,437 4 View

how could a badly designed primer pair affect a PCR (conventional and quantitative)?

04 May 2014 4,800 3 View

Which free journals are there in topics of molecular microbiology with impact factor between 1 and 2?

Our study investigated a real time PCR technique as a diagnostic tool, and for virulotyping (screnning of virulence genes) by real time PCR; can anyone suggest some journals (free of charges plz)...

01 February 2014 4,637 5 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View