How to re-cluster PFGE restriction profiles from gel pictures noting that i do not have access to bionumerics?

We have the results of pulsed field gel electrophoresis PFGE of some bacterial strains , and we clustered bacterial strains with bionumerics in fact, we are no more able to use the software since...

31 December 2018 409 0 View

S1 PFGE for visualizing megaplasmids, what differs from a ususal PFGE (other than using S1 instead of res enzyme)?

The question is about S1 PFGE ; a pulsed field in which S1 is used to linearize plasmidic DNA so that the latter could migrate through pulsed elecrophoresis. i intend to run a usual PFGE, i wanted...

02 March 2018 1,037 2 View

Why use mineral oil in RAPD experiments and which one?

It is indicated that RAPD reactions are made with a layer of mineral oil on the top of the mixture, iI would like to ask which oil to use and how and why it is used? I actualy did the experiment,...

09 October 2014 2,437 4 View

how could a badly designed primer pair affect a PCR (conventional and quantitative)?

04 May 2014 4,800 3 View

Which free journals are there in topics of molecular microbiology with impact factor between 1 and 2?

Our study investigated a real time PCR technique as a diagnostic tool, and for virulotyping (screnning of virulence genes) by real time PCR; can anyone suggest some journals (free of charges plz)...

01 February 2014 4,637 5 View

A question about your choice. Why wild animals?Are you searching just for financial support or you need cooperating laboratories to do the analyzis?

no details needed

01 January 1970 4,404 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View