How to quickly induce a hepatocellular carcinoma in a rat?

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Can someone please give me this protocol in detail?

LDH activity in serum was determined kinetically according to Buhl and Jackson.,(1978). Principle: pyruvate + NADH+H LDH lactate + NAD+ -the rate of NADH oxidation...

04 May 2014 4,059 1 View

How can I calculate the optical density of supernatant and pellets using DNA fragmentation?

Diphenylamine (DPA) assay: DNA fragmentation in liver tissue will be carried out according to Perandones et al. (1993). Principle: A quantitative method was raised to determining % of...

04 May 2014 1,508 2 View

Can anyone help with the determination of glutathione reductase in liver homogenate according to Beutler (1975)?

I'm looking for the determination of glutathione reductase in liver homogenate according to Beutler 1975 and how I can gain the result in micromole/gram tissue.

03 April 2014 2,559 1 View

Can anyone help me with the determination of catalase enzyme in liver homogenate using Claiborne (1985)?

I'm looking for the determination of catalase enzyme in liver homogenate using Claiborne 1985 and how I can obtain the result in U/mg protein.

03 April 2014 4,323 5 View

What are the normal values of Superoxide dismutase, glutathione peroxidase, reduced glutathione and malinoaldehyde in tissue of rat?

I want to know normal value of Superoxide dismutase and glutathione peroxidase and reduced glutathione and malinoaldehyde in tissue of rat.

02 March 2014 7,131 5 View

Can glass affect the colour of liver enzymes in serum frozen for 21 days in test tubes?

Also, does freeze storing for a prolonged period affect the measurement of AST ALT GGT LDH?

31 December 2013 5,977 2 View

How to convert a toxic dose for a zebrafish to be equivalent to the one for rats?

I want to use the toxic dose for a rat. I want to ask if I can use the dose without changing anything or do I need to make a certain conversion of the dose for zebrafish to be equivalent to the...

09 October 2013 3,129 9 View

What is the meaning of dispersion of silver nanoparticles?

What is the meaning of dispersion and the importance of dispersion of silver nanoparticles in citrate?

08 September 2013 7,341 5 View

Is water a suitable solvent for curcumin?

Is water suitable as solvent to curcumin to use oral in vivo?

07 August 2013 5,568 35 View

Can anyone explain how liver rich with thiol group?

How liver rich with thiol group

07 August 2013 7,367 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Alex Boye

You can try with carbon tetrachloride or indomethacin or better still check pubmed central for some articles which had used some models.

Jyoti Srivastava

You can try DEN 'diethylnitrosamine' treatment. We have used this carcinogen to induce HCC in mice and you can see my paper appeared in hepatology for dose and time. If you have any question, let me know..

Best of luck,

Jyoti