How to quickly induce a hepatocellular carcinoma in a rat?

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Can someone please give me this protocol in detail?

LDH activity in serum was determined kinetically according to Buhl and Jackson.,(1978). Principle: pyruvate + NADH+H LDH lactate + NAD+ -the rate of NADH oxidation...

04 May 2014 3,992 1 View

How can I calculate the optical density of supernatant and pellets using DNA fragmentation?

Diphenylamine (DPA) assay: DNA fragmentation in liver tissue will be carried out according to Perandones et al. (1993). Principle: A quantitative method was raised to determining % of...

04 May 2014 1,426 2 View

Can anyone help with the determination of glutathione reductase in liver homogenate according to Beutler (1975)?

I'm looking for the determination of glutathione reductase in liver homogenate according to Beutler 1975 and how I can gain the result in micromole/gram tissue.

03 April 2014 2,487 1 View

Can anyone help me with the determination of catalase enzyme in liver homogenate using Claiborne (1985)?

I'm looking for the determination of catalase enzyme in liver homogenate using Claiborne 1985 and how I can obtain the result in U/mg protein.

03 April 2014 4,233 5 View

What are the normal values of Superoxide dismutase, glutathione peroxidase, reduced glutathione and malinoaldehyde in tissue of rat?

I want to know normal value of Superoxide dismutase and glutathione peroxidase and reduced glutathione and malinoaldehyde in tissue of rat.

02 March 2014 7,058 5 View

Can glass affect the colour of liver enzymes in serum frozen for 21 days in test tubes?

Also, does freeze storing for a prolonged period affect the measurement of AST ALT GGT LDH?

31 December 2013 5,918 2 View

How to convert a toxic dose for a zebrafish to be equivalent to the one for rats?

I want to use the toxic dose for a rat. I want to ask if I can use the dose without changing anything or do I need to make a certain conversion of the dose for zebrafish to be equivalent to the...

09 October 2013 3,059 9 View

What is the meaning of dispersion of silver nanoparticles?

What is the meaning of dispersion and the importance of dispersion of silver nanoparticles in citrate?

08 September 2013 7,284 5 View

Is water a suitable solvent for curcumin?

Is water suitable as solvent to curcumin to use oral in vivo?

07 August 2013 5,509 35 View

Can anyone explain how liver rich with thiol group?

How liver rich with thiol group

07 August 2013 7,309 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Where to find data on trees in tropical agroforestry?

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28 February 2021 7,356 8 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Alex Boye

You can try with carbon tetrachloride or indomethacin or better still check pubmed central for some articles which had used some models.

Jyoti Srivastava

You can try DEN 'diethylnitrosamine' treatment. We have used this carcinogen to induce HCC in mice and you can see my paper appeared in hepatology for dose and time. If you have any question, let me know..

Best of luck,

Jyoti