LDH activity in serum was determined kinetically according to Buhl and Jackson.,(1978).
Principle:
pyruvate + NADH+H LDH lactate + NAD+
-the rate of NADH oxidation is proportional to LDH activity.
Calculation:
-an international enzyme unit (U) is defined that activity of enzyme that convert one micromole of substrate in one minute under standard conditions.
Formula:
Enzyme activity(U/L) =
D A / Min.X (Vt x 1000)/(MEC X LP X VS)
=D A / Min x 8200 (at 340 nm and 1 cm light path)
Where
*U/L = international unit per liter.
*Vt= total volume of reaction (ml).
*1000= convert concentration to liter.
*MEC = micromolar extinction coefficient of NADH (6.22)
*LP = light path (cm).
*Vs= sample volume (ml).
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