Mohamed Fathi

13 Questions 7 Answers 0 Followers

Questions related from Mohamed Fathi

How can I calculate the optical density of supernatant and pellets using DNA fragmentation?

Diphenylamine (DPA) assay: DNA fragmentation in liver tissue will be carried out according to Perandones et al. (1993). Principle: A quantitative method was raised to determining % of...

05 May 2014 1,465 2 View

Can someone please give me this protocol in detail?

LDH activity in serum was determined kinetically according to Buhl and Jackson.,(1978). Principle: pyruvate + NADH+H LDH lactate + NAD+ -the rate of NADH oxidation...

05 May 2014 4,029 1 View

Can anyone help with the determination of glutathione reductase in liver homogenate according to Beutler (1975)?

I'm looking for the determination of glutathione reductase in liver homogenate according to Beutler 1975 and how I can gain the result in micromole/gram tissue.

04 April 2014 2,525 1 View

Can anyone help me with the determination of catalase enzyme in liver homogenate using Claiborne (1985)?

I'm looking for the determination of catalase enzyme in liver homogenate using Claiborne 1985 and how I can obtain the result in U/mg protein.

04 April 2014 4,272 5 View

What are the normal values of Superoxide dismutase, glutathione peroxidase, reduced glutathione and malinoaldehyde in tissue of rat?

I want to know normal value of Superoxide dismutase and glutathione peroxidase and reduced glutathione and malinoaldehyde in tissue of rat.

03 March 2014 7,096 5 View

Can glass affect the colour of liver enzymes in serum frozen for 21 days in test tubes?

Also, does freeze storing for a prolonged period affect the measurement of AST ALT GGT LDH?

01 January 2014 5,951 2 View

How to convert a toxic dose for a zebrafish to be equivalent to the one for rats?

I want to use the toxic dose for a rat. I want to ask if I can use the dose without changing anything or do I need to make a certain conversion of the dose for zebrafish to be equivalent to the...

10 October 2013 3,087 9 View

How to quickly induce a hepatocellular carcinoma in a rat?

I want to know the methods to induce hepatocellular carcinoma in rat in a fast way

09 September 2013 10,075 2 View

What is the meaning of dispersion of silver nanoparticles?

What is the meaning of dispersion and the importance of dispersion of silver nanoparticles in citrate?

09 September 2013 7,311 5 View

Can anyone explain how liver rich with thiol group?

How liver rich with thiol group

08 August 2013 7,337 1 View

Is water a suitable solvent for curcumin?

Is water suitable as solvent to curcumin to use oral in vivo?

08 August 2013 5,539 35 View

What is the effect of silver nanoparticles on myoglobin?

And if there is effect of corona (nanoparticles-protein interaction) play role in that?

07 July 2013 9,147 2 View

Toxic dose of silver nanoparticles

I intend to inject silver nanoparticles intraperitoneal in rat with dose 100 and 200 micromole. I want to know if these doses are sufficient to induce toxicity in rat without killing the rat?

05 May 2013 9,805 1 View