I want to use the toxic dose for a rat. I want to ask if I can use the dose without changing anything or do I need to make a certain conversion of the dose for zebrafish to be equivalent to the one for rats?
You can also check from literature for existing reports for your chemical of interest.One should follow the OECD Guidelines to find the toxic dose and as the response varies in different biological systems. The dose is also administered with respect to the body weight. as you perform the preliminary acute toxicity studies according to the guidelines you can standardize the concentration. this link might help you... http://www.oecd.org/chemicalsafety/risk-assessment/1948378.pdf.
The dose that i will use 100 micromole it is toxic in zebrafish i want to use the same dose if possible to rat intraperitoneal
I also have similar doubts.
Does the toxicological study in zebrafish are equivalent? If not how to compare?
I need to study toxicological effect of some XYZ, if I have studied the same in zebrafish then can the both comparable?
Zebrafish and rat have totally different physiology and most probably will respond differently to a chemical. In our Institute people get totally different results to a chemical stimulus in marine and fresh water fishes. I think you would have to standardize your dose in rat. Good luck sir.
You can also check from literature for existing reports for your chemical of interest.One should follow the OECD Guidelines to find the toxic dose and as the response varies in different biological systems. The dose is also administered with respect to the body weight. as you perform the preliminary acute toxicity studies according to the guidelines you can standardize the concentration. this link might help you... http://www.oecd.org/chemicalsafety/risk-assessment/1948378.pdf.
Uhmmm I don't think there is a direct way to convert toxic doses between species. Each species have their own peculiar physiology. You'll need to standardize your dose in each species then, calculate your toxicological parameters for each species and then compare. Please be very careful when interpreting your data because usually zebrafish is exposed to toxicants in water and you don't have a "dose" but an exposure concentration.
I agree with Gabriela above. It is not possible to convert the rat dose to a zebrafish dose as the methods of administration are different. In the past I have looked at relative toxicities between different treatments within one model (zebrafish or rat) e.g. chemical X is the most toxic out of X, Y, and Z for both zebrafish and rats. However it's not possible to directly compare g/g toxin/body weight to g/L toxin/solution concentration.
I agree with the comments above and also suggest reading: http://dx.doi.org/10.1016/j.cbpc.2008.07.013 which handles this topic nicely comparing rats and medaka (similar aquarium model).
Thank you all of you. Its very fruitful discussion which is really so important to everyone. I facing the same question to myself but I was unable to came out with my answer. Based on my experience, we really don't need to convert dose for zebrafish to animal or human. However, it will not be more accurate like other species to human dose (Rat, dog, rabbit, mice and pig) because the authority like FDA or OECD have clear guideline for this but no guideline for dose conversion of zebrafish to animal or zebrafish to human. Its just screening for toxicity of our tested or targeted chemicals. I hope that's all. Thank you.
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