I have got FTIR spectra of natural fibers and would like to know the surface concentration of lignin and cellulose. Is there any method for quantitative calculation for different components based on FTIR? How?
Difficult issue...
There has been work done on Wood for mapping Cellulose/Lignin distribution and relative concentrations. You can find literature on this, using FTIR, Raman imaging. But the lateral XY resolutions are much below those you would need for single fibers.
You can still try FTIR imaging, but using ATR-Imaging (e.g. with an imaging ATR attachement onto an imaging IR microscope) : lateral resolution limit would be ca. 4 µm. Not many points across a wood fiber's diameter, even less for some annual plants fibers.
Raman imaging has reportedly lateral resolutions that can go below the micrometer but I have no experience with that (would love to...).
Afterwards, it's a "chemometrics" issue : you would need to try various techniques (band ratio maybe if your spectra are resoluted, PCR...).
Another suggestion (off the top of my head) : functionalized SPM/AFM ?
bit of confusion as you try to figure out some quantitative info from basically a qualitative datasheet, you can try a passive way, firstly get a FTIR of non-modified fiber, and then compare the intensity of the desired functional group of your modified fiber.
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