no
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Is there any protocos to produce the medium for single hybridoma cell culture without the feeder cells layer in the plate(Co-culture)?
20 May 2024 8,214 2 View
The development of mRNA vaccines on mice and rabbits?
18 May 2024 7,475 2 View
We have constructed lentivirus transduced U251 cell lines to express (1) EGFRvIII (its amino acid sequence is MRPSGTAGAAFLALLAALCPASRALEEKKGNYVVTDHG..., which is identical to the sequence...
14 April 2024 2,370 1 View
NO
11 March 2024 3,725 1 View
It is a common belief that extreme weather and climate events increased in frequency and intensity over the last century or half a century, a time period that saw a rapid global climate warming....
16 February 2024 3,727 10 View
Does anyone know where I can find the data of measured above-ground biomass? I need the data of forest biomass measured on the spot for training and learning. If possible, I can also invest money...
12 November 2023 7,845 3 View
How can we achieve the effect of the added magnetic field on the electrolysis process during molten salt electrolysis?
01 September 2023 4,416 2 View
I'd like to know what's current progress of 'synthetic circadian clock' or 'synthetic oscillators'. Indeed, I would like to investigate methods to regulate protein expression (e.g., transcritpion)...
24 August 2023 8,165 1 View
Weather stations are key facilities to record long-term change in surface air temperature (SAT) in land. However, some weather stations are located in or near cities, and they may suffer from...
20 June 2023 8,860 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
EDX analisys
31 July 2024 5,947 2 View
I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...
30 July 2024 969 0 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
