How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

What is the difference between the supplementary cementitious materials and alkali-activated concrete?

18 February 2021 9,188 3 View

For displacement control test, what is the relation between stress intensity factor and crack length?

There are two plates with horizontal center crack, one with crack length of a with the other with a+da. The bottoms are fixed while the tops are under the same displacement load. For intuition,...

11 February 2021 9,543 2 View

Difference between Second-harmonic generation and Two-photon absorption?

Hello Dear Researchers, I am confused about the Difference between Second-harmonic generation and Two-photon absorption? Can anyone describe the two phenomena briefly in the physical picture? What...

24 January 2021 9,912 5 View

Which kind of GPC solvent can be used to dissolve both lipid and salt?

I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).

14 January 2021 3,805 2 View

Is it possible to publish a paper contradicting Einstein's special relativity in any Journal?

I just developed a new theory on electromagnetic and plan to publish in a Journal. The findings are purely through strict mathematical derivation without any disputable assumptions and also...

13 January 2021 1,558 5 View

How is linear form of plasmid circularized?

Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...

13 January 2021 8,336 4 View

Why dose plasma AngII concentration decrease in AngII infusion mice detected by AngII ELISA kit?

I detect the plasma AngII concentration by AngII ELISA kit (ENZO), the results show that plasma AngII decrease in AngII infusion mice. I check with the kit and find that the kit could recognize...

31 December 2020 8,838 3 View

How can I downgrade Networkx 2.5 to 2.3 version?

When I run the command according to the GROMACS tutorial, I got the below result This script has been tested with NetworkX 2.3, and 2.4 is buggy. Please install version 2.3 for best...

27 December 2020 4,699 3 View

FoldX YASARA plugin keep showing error message. Please help?

Hi, I use YASARA (version 17.8.15) on windows 10 and recently I am trying to install FoldX plugin on YASARA. Here is what I did: 1. Download YASARA plugin for windows (and the executable and...

24 December 2020 536 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View