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I purified the protein with Hi-trap column, the final buffer is 25mM HEPES, 500mM NaCl, 500mM imidazole. However, after overnight dialysis, the protein always precipitate. Anyone can help me to...
08 September 2019 7,291 7 View
using his-tag column to purify. the condition for wash step is 40mM imidazole. and using gradient concentration of elution buffer from 60mM to 250mM. the final product only got 75%. is there any...
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