Can somebody suggest sample preparation protocol for FT-IR and SEM analysis for Inclusion body protein?

I want to do structural characterization of my recombinant protein which is going into inclusion body

15 January 2020 6,762 1 View

From where can I procure colloidal carbon nanoparticles in India?

I am aiming to use carbon nanoparticles in my immunochromatography assay & most of the reference paper have use Colloidal carbon particles ( Spezial Schwartz 4 [SS4]; Degussa AG, Frankfurt,...

26 March 2019 9,077 3 View

What are the best practices to model structure of an insect olfactory / odorant receptor (computationally) according to you?

Kindly suggest your views on the topic. Please provide references wherever possible.

18 August 2017 5,106 7 View

What exactly is the concept of antibody pair with respect to lateral flow assay?

30 July 2017 4,972 3 View

What are the markers to identify higher milk secretion in the mammary glands of lactating Wistar rats?

I am studying the histology of mammary glands of lactating Wistar rats. I need to differentiate glands with higher milk secretion from the glands with lesser or no milk secretion. What are the...

21 March 2017 3,172 1 View

How can we determine the molecular weight of an inorganic polymer?

I want to determine molecular weight of poly aluminum chloride.

01 May 2014 3,760 2 View

Fermentation technology involved with product processing.

I need some research resources on downstream prossesing purification & pakaging, drying,recovery of fermentation products.

26 February 2012 7,230 12 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View