Snehal Ganjave

3 Questions 2 Answers 0 Followers

Questions related from Snehal Ganjave

pET32b for protein expression?

Can we utilize the N-terminal His-tag of pET32b vector for His tag purification? I have incorporated the stop codon in my reverse primer. Thank you.

11 August 2021 4,388 2 View

How to purify Inclusion body Protein with higher pI?

I want to purify my inclusion body protein by using AKTA. pI of the protein is 9.4. Can Someone help me which column should I select ? CM sepharose or SP? Please suggest me the procedure. Thank you.

13 May 2021 4,241 0 View

Can somebody suggest sample preparation protocol for FT-IR and SEM analysis for Inclusion body protein?

I want to do structural characterization of my recombinant protein which is going into inclusion body

16 January 2020 6,849 1 View