I have to treat mitochondria with rotenone in aiming to induce Reactive oxygen species (ROS) and by antioxidant prevents it's ROS generation.
Therefore,by In-vitro model how can I take the volume needed for each
1) mitochondria volume?
2)rotenone volume?
3) antioxidant volume?
For mitochondrial treatment. If we know their molar concentration only?
For example
1)Control- (mitochondria)
2) Mitochondria+ rotenone+ antioxidant.
To treat mitochondria in vitro with rotenone and an antioxidant, you can use the following protocol:
Example:
It's important to note that these volumes may need to be optimized for your specific experimental conditions and that it may be helpful to perform pilot experiments to determine the optimal concentrations and volumes of rotenone and antioxidant for your particular experiment.
Dear Geir Bjorklund ,
Your answer was very much helpful for my research work.
In addition, I would like to ask that after mitochondrial isolation although we are doing the protein estimation for its characterization, how can we test whether our mitochondria will be viable or not?
or which assay do we have to go through?
Dear Geir Bjorklund ,
could you please share with me any research paper related to my research question? for invitro ROS study on rat brain/liver mitochondria, with Sodium Nitroprusside/ rotenone as a inducer?
Thanking you
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs