Hello, I'm working on Potato psyllid. For checking gene expression lever, I used to dissect guts. But I'm always worried about the quality of RNA. Currently, I'm using RNA later with 1X PBS buffer for dissecting guts. After dissecting 20 ~ 30 guts, I removed this mix and started RNA extraction. If you have better way for this, please answer me.
Mª Angeles Zorrilla Lopez-Perea
I will try. Do you mean just on the protocol or the extraction? RNA has always been very delicate. The buffers do help mantain the RNA, however, maybe in a crude way might pose a problem. The crude RNA might lose properties, maybe proteases and stabilizers to keep away any proteins. Anything to mantain the RNA the better. Sometimes it might pose a problem.
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