Junepyo Oh

3 Questions 3 Answers 0 Followers

Questions related from Junepyo Oh

How to mount insect tissues (brains or guts) on microscope slide?

Hi, I'm trying to take picture after immunolocalization with insect guts. After I did all process, I have a problem when I mount the guts. I used pipette and pipette tip (I cut the tip) to do...

27 January 2022 4,320 3 View

How do I amplify the genes from yeast cell using yeast colony pcr (yeast two hybrid) ?

Hello, I'm working on yeast two hybrid. After I mating, I usually do yeast colony pcr to amplify AD plasmid. If I test 50 yeast colonies from quadruple dropout media. I barely get 10 amplicons. I...

26 October 2021 2,105 2 View

How to preserve or stabilize RNA during dissecting process from insect guts?

Hello, I'm working on Potato psyllid. For checking gene expression lever, I used to dissect guts. But I'm always worried about the quality of RNA. Currently, I'm using RNA later with 1X PBS buffer...

23 September 2021 7,434 1 View