Nowadays I am working on tissue culture (CEF) which i infected with virus and i want to preserve some of these CEF for future work to detect if it was infected by this virus or not and to measure the quantity of these viruses using Reai time PCR or for more further examination of genome of these viruses.
generally want the procedures to preservation of an infected tissue culture
If you're just looking at nucleic acid information, you should be able to freeze it at -80C. I'm pretty sure you can just freeze the culture in cyrovials without even processing them to harvest the RNA or DNA first. However, if you're only looking at RNA and DNA, it might be wise to go ahead and extract those and then freeze them for later analysis. RNA won't last as long as DNA. You should be good for at least a month if you freeze the culture without processing, a few months if you harvest the RNA and at least a year if you harvest the DNA.
thank you very much hans for your answer, for me i am looking for a specific gene on the DNA of the virus, and i freeze the medium with the infected cell into -80 without processing and I hope this process will be effective with my work
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