Mohamed S Ahmed

6 Questions 22 Answers 0 Followers

Questions related from Mohamed S Ahmed

How do I prepare a primer of 100 µM stock solution?

Today i received a GAPDH- AB, MdCv-F and SNV-LTR-F/R primers forward and reverse and this is the first time for me to make a PCR reaction and I think I need to prepare a 100 µM stock solution...

03 February 2015 3,222 7 View

How to preserve infected CEF (chicken embryo fibroblast) tissue culture for further molecular examination?

Nowadays I am working on tissue culture (CEF) which i infected with virus and i want to preserve some of these CEF for future work to detect if it was infected by this virus or not and to measure...

06 August 2013 7,897 2 View

How I can get rid of the fungal contamination?

I have fungal contamination in my incubator and I want anything that may help me to get rid of this contamination because it's bothering me in cell culture or any work inside incubator. I washed...

25 July 2013 8,793 22 View

What is the procedures to infect a healthy cell culture with another viral infected cell culture?

I have 5 dishes of Chicken embryo fibroblast (CEF) culture infected with virus and I want to infect another new CEF by the previously infected cell culture. Could someone tell me how to do this...

15 July 2013 8,297 2 View

What could cause the detachment of Chicken embryo fibroblast (CEF) inoculated with MD virus?

I inoculated MD virus into CEF but the I found the cell sheet was detached in one of my dishes

15 July 2013 1,555 3 View

Is there any available cell line or tissue culturing for Psittacine beak and feather disease?

This is a viral disease belonging to family Circoviridea and it considered the smallest viral particle among the avian pathogens. Until now it had been difficult to find a cell line for its...

14 February 2013 9,436 2 View