I wanted to calculate ic50 of mebendazole against a549 cancer cell lines. I have prepared 10mM main stock in DMSO (completely dissolved) but when I am trying to make 50uM, 25uM, and 10uM working stock in media/PBS (keeping DMSO concentration less than 1%), I am getting precipitate. The mebendazole is not getting dissolved.

Note:- The mebendazole is from cayman and it's 99.9% pure.

kindly, help me in this regard that how to prepare a working concentration of mebendazole to get a complete dissolved solution of it in media/PBS, or I have to use any other solvent.

Thanks in advance.

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