How to prepare the working solution of cotrimoxazole for MIC test?

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What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Any recommendations for tetR antibody for IHC?

I am planning an experiment where I've to detect cells which are expressing TetR, my vector is so big that I can't have a reporter in it. I was thinking if I can detect the expression of TetR...

24 September 2020 1,041 4 View

How do I reduce the size of a file in Comsol simulations?

I am working with RF module in comsol and doing parametric sweeps in frequency domain. The data generated is huge and taking a lot of space on my disc. The fine meshing that I need for my problem...

22 July 2020 4,374 6 View

How to get the antenna to resonate at the required frequency with the ideal magnitude in the s-parameter graph?

I have designed a rectangular patch antenna for a frequency of 2.4Ghz using Rogers 5880 as my substrate material with a thickness of 1.6mm. But, my s-parameter graph does not show a graph with the...

03 October 2019 6,543 4 View

Best reagent for Colorimetric detection of chloride ions?

I want to find an assay for colorimetric method of detection of chloride ions from blood or body fluids. The main issue is I cannot use mercury or mercury derived chemicals in my assay. Can...

04 February 2019 8,093 3 View

How to grow N.gonnorhea?

02 August 2016 1,264 1 View

I tried extracting plasmid DNA by Brimbroim Dolly method. After running on agarose gel I am getting mutiple bands of different molecular weights.why?

Are all the bands plasmids itself?or is it degraded plasmid?

30 July 2016 7,069 11 View

What can be the possible outgroup for Ryphilla sp (crab, leucosidae) while drawing phylogenetic tree with Co1 gene??

Which all organisms can be chosen as best outgroups for Ryphilla crabs in the phylogenetic tree

30 July 2016 9,087 1 View

Can you provide any method for APEO analysis on HPLC?

bufffer of what pH can we use as mobile phase?

01 March 2016 8,861 1 View

Can anyone please send me the procedure for plasmid extraction from Vibrio species?

11 February 2016 1,768 2 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

What is role of toluene and stearic acid in ball milling for powder ? In what weight percent we should add this to powder?

I am doing ball milling operation of Aluminum and silicon carbide powder. I am pretty confused about what should be added as process control agent ? what is role of toluene as PCA and stearic...

12 February 2021 5,205 3 View