I need to use pantothenate as supplement for culture mc2 6020 and mc2 6030.
the pantothenate powder was diluted in MiliQ(water), then filtered with 0.2 um filter, I often find pan contaminated by other bacteria.
Dear Lihui,
Sterilization through 0.2 um should do the job, however, you may used glass ware or containers that were not sterilized.
The following is a procedure to get sterile pantothenate aliquot:
Pantothenic acid (FW 238.3 hemicalcium salt): Dissolve 0.405 g pantothenic acid in 100 ml water and filter sterilize to yield 17 mM (1000X) stock. Dispense 2 ml aliquots in sterile cryovials and store at -20°C or below; thawed stock can be kept at 4°C for up to 1 week.
https://catalog.coriell.org/0/pdf/1401_38.pdf
Hoping this will be helpful,
Rafik
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