I need to immunize mice subcutaneously with 500 ug OVA in Complete Freund's Adjuvant. How should I prepare the mix to do it correctly?
I'm sorry but I do not have experience in your area.
Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...
22 July 2024 5,492 6 View
I am writing a paper summarizing my research results on the possibility of usage of volcanic ash in modern cementites materials on the example of the tests of composits with particular (Japanese)...
21 February 2024 5,797 0 View
I am trying to calculate DBH (diameter at breast height) in order to determine the demography and basal area of trees. However, some of the trees have multiple stems which makes it difficult to...
23 January 2024 845 0 View
The site shows that my works are cited in some papers, however when I checked those papers, I found no sign of being referenced (on the other hand my work is referenced in completely different...
22 January 2024 3,766 2 View
I'm looking for hard-working researchers for the new research project "EDUCATION IN THE AI ERA." I would like to create the survey on Google and do international research. If you are interested in...
06 January 2024 5,488 21 View
Hi everyone, I'm currently working with a plasmid called pBI101, I isolated this plasmid from a plate then I did the extraction with mini prep protocol and cut it with SmaI at 25°C incubation...
17 November 2023 6,980 3 View
I'm trying to optimize TraDIS for our model bacterium and I am having some technical problems with the DNA preparation for the sequencing step. I'm struggling with efficient PCR enrichment of...
12 September 2023 5,836 0 View
Hello everyone, I am trying to make a CRISPR-Cas9 knockout on lung cancer primary cells using the RNP complex. I am using the Neon Transfection System but so far it failed - all the cells that...
30 August 2023 8,660 2 View
Hi, does anyone know the formula for converting the BDNF value in biological fluids (pg/ml to ng/ml and vice versa)?
24 July 2023 1,280 1 View
Hi, I'm doing CFA using SPSS Amos and I have two variables with single items each. After reviewing previous discussions, I've fix the loadings and error variance to 1,1. If I put error variance to...
24 June 2023 3,631 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Greetings all, I am a masters student and I want to improve my understanding of confounding variables, especially in cross sectional studies. i have read quite a lot about it, and I know that...
25 July 2023 8,573 0 View
Hello, everyone. recently I've been trying to establish passive EAE model by transfer Th17 cells polarized in vitro (from 2D2 mice). according to previous papers (like Ja¨ ger et al., 2009 ), 2d2...
19 January 2022 3,217 1 View
"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...
11 February 2021 7,120 9 View
Recent studies have highlighted the isolation of a new strain of SARS-Cov-2. Could the new discovery compromise the effectiveness of the vaccination campaign ???
21 December 2020 7,881 4 View
If possible, let us know about the which vaccine would be available in your geographic location.
06 December 2020 3,940 23 View
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/5/s6322dat.pdf Has anyone prepared a vial of sigma adjuvant systems (Ribi's) when they either aren't using the whole...
02 September 2020 7,908 2 View
I am searching for some literature regarding the maximum tolerable concentration of metal ions that doesn't develop any toxicity and/or unwanted immunogenicity in albino mice
24 July 2020 3,075 0 View
I had a few questions regarding Convalescent Plasma Therapy Why can't we use whole blood rather than convalescent plasma only? or Plasma + WBC (Adoptive cell transfer). This way we will transfer...
10 June 2020 2,764 9 View
I want to perform splenocyte restimulation culture for the estimation of Th1 and Th2 cytokines by ELISA. As each groups consists of 5 mice immunized with a test vaccine preparation; can splenocyte...
08 September 2019 4,812 2 View