How to prepare OVA in CFA?

10 December 2013 1 8K Report

I need to immunize mice subcutaneously with 500 ug OVA in Complete Freund's Adjuvant. How should I prepare the mix to do it correctly?

Sharon Frentzel

I'm sorry but I do not have experience in your area.

Badges
Science topic

More Joanna Marczyńska's questions See All

How to test genetic stability of bacteria?

Hi! I'm looking for a paper or a protocol of a validated method for testing of genetic stability of bacteria. Does anybody know sth like that? We were looking for the papers on this subject but we...

12 January 2021 490 8 View

Is there any easy way to check if given miRNA hybridize to RNA (extracellularly)?

Suppose we have given miRNA (that according to in silico analysis should bind to a specific sequence in RNA) that is labeled with a fluorescent dye (or is labeled otherwise) and we have isolated...

12 November 2020 5,188 1 View

Is anyone interested to join me to the Erasmus project?

I'm Erasmus coordinator from Poland. I'm open to cooperate with another coordinator within Erasmus project. If you are looking for the team, please write to me. Joanna

18 July 2020 7,910 2 View

Is anyone interested in collaborating on an article in the field of effective time management?

I'm looking for someone that is interesting collaboration in effective time management area.

15 July 2020 9,068 5 View

Has anyone done research what are the determinants of implementing industry 4.0 solutions from the point of view of PESTEL factors?

I am looking for research results, scientific articles, tips which factors affect on Industry 4.0 implementation and spread of using in practice. Maybe simple PESTEL factors could be useful -...

17 June 2020 7,494 1 View

What statistical analysis would you recommend to use for my survey data?

Hi everyone, I am currently doing my dissertation for MSc in Strategic Management. My topic focuses on the impact of social media on customer service: case study on Twitter accounts. I have...

24 May 2020 5,275 5 View

What scales could be used to measure wellbeing during the coronavirus pandemic?

I'm looking to find some outcome measures that could address how people feel they are able to cope psychologically during the pandemic and wondered if anyone had any thoughts? Maybe something...

30 April 2020 8,316 3 View

Do I need to address temporal autocorrelation when using boosted regression trees?

I am using boosted regression trees (BRT) to investigate the environmental influences of shark presence at a single location. I have a year-long dataset of acoustic tag detections and...

28 April 2020 914 4 View

Could you help with a survey that concentrates on contact lens behaviours and opinions during COVID-19 pandemy?

Please see the attached invitation to the survey.

25 April 2020 2,635 3 View

What is the possible reason why MM calculation of a protein in Spartan suddenly stopped computing?

I am currently running MM (MMFF94) of a protein-ligand complex for geometry optimization using Spartan '18. The calculation went smoothly until the 19919th cycle when it suddenly stopped computing...

19 March 2020 3,628 4 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

Will the spread of the new SARS-Cov-2 strain affect the efficacy of mRNA-based vaccines?

Recent studies have highlighted the isolation of a new strain of SARS-Cov-2. Could the new discovery compromise the effectiveness of the vaccination campaign ???

21 December 2020 7,516 4 View

When available, are you planning to receive SARS-CoV-2/COVID-19 immunization? What do you most fear about it?

If possible, let us know about the which vaccine would be available in your geographic location.

06 December 2020 3,527 23 View

How to prepare a vial of Sigma Adjuvant System (Ribi) when needing multiple immunogens/at different immunisation points?

https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/5/s6322dat.pdf Has anyone prepared a vial of sigma adjuvant systems (Ribi's) when they either aren't using the whole...

02 September 2020 7,451 2 View

Regarding Convalescent Plasma Therapy for COVID-19?

I had a few questions regarding Convalescent Plasma Therapy Why can't we use whole blood rather than convalescent plasma only? or Plasma + WBC (Adoptive cell transfer). This way we will transfer...

10 June 2020 2,361 9 View

Is it acceptable to mix splenocytes isolated from two different mice immunized with same antigen?

I want to perform splenocyte restimulation culture for the estimation of Th1 and Th2 cytokines by ELISA. As each groups consists of 5 mice immunized with a test vaccine preparation; can splenocyte...

08 September 2019 4,493 2 View

% population of CD4 CD25 and FOXP3 for Control Vs Test groups?

After some reading and checking for the Treg cells during helminthic infection, I found my self somewhat confused for the % populations measured for CD4 CD25 and FOXP3 for Control against Text...

18 March 2019 6,915 3 View

Could e-card for immunisation be the innovation that could improve completion of immunisation among under 5 in the developing country?

There are challenges facing parents in keeping paper immunisation card in maintaining proper vaccination records for children under 5 years. The parents are faced with multitude of problems and...

27 January 2019 6,956 2 View

Could e-card for immunisation be the innovation that could improve completion of immunisation among under 5 in the developing country?

There are challenges facing parents in keeping paper immunisation card in maintaining proper vaccination records for children under 5 years. The parents are faced with multitude of problems and...

31 December 2018 936 2 View

What is the routine immunization proxy Index ? and how to determine this index?

In a country the Immunization coverage is quite low. During discussion it was suggested to calculate the Routine Immunization proxy Index. I would like to know what this index stand for, the...

10 November 2018 5,296 0 View