Hello, everyone. recently I've been trying to establish passive EAE model by transfer Th17 cells polarized in vitro (from 2D2 mice). according to previous papers (like Ja¨ ger et al., 2009 ), 2d2 CD4 naive T cell should be isolated and then co-culture with APCs in the presense of Th17-differentiating cytokines and MOG35-55. Here what is the kind of APC? (many paper didn't mention). Would you like to choose CD11c+ cells?. By the way, sometimes it should be irradiated APC. what's the condition of irradiaiton and the cell types of APC?