Beta value calculation of absolute intracellular calcium concentrations?

Hi all, I am doing calcium imaging experiments in N2a cells and wanted to calculate the absolute calcium concentrations. Can someone help me out in figuring out how to calculate the fluorescence...

24 March 2020 7,974 1 View

What is the significance of 'dark spectral response'?

Can anyone explain me the significance of ''dark spectral response'' or " Dark EQE" of solar cell and how it will help to improve the efficiency of solar cell?

17 April 2019 7,021 6 View

What can I find out by performing DSC on a polymer sample? Also how would I interpret a curve that I obtained?

Metallurgical engineer who has never performed DSC and now I am working with polymers. Am able to do the test on the equipment however I am still facing troubles with interpreting the curve that I...

06 March 2018 7,660 4 View

Which polymer can be in rich in surface when a mixture of two hydrophobic polymers coated on substrate?

"A" is the polymer having low molecular weight and low surface energy. "B" is the polymer with high molecular weight and high surface energy. In a mixture "B" is slightly large concentration than...

31 October 2016 1,742 2 View

How to avoid this warning in Silvaco?

No ionization integrals possible at this bias point. Possibly applied voltage is less than DELTAV in field line calculation or the electrode does not contact any semiconductor material.

20 October 2016 8,761 2 View

How do I select the best primer which does not produce any non specific bands from those I have designed?

Hi! I am currently designing primers for the human UGT1A1 gene using the Primer3 software. However, when I BLAST the primers against the Human RefSeq database, I get significant matches with other...

10 October 2016 7,172 3 View

How to perform enzyme activity assays?

If i want to perform any enzyme activity how to select the substrate concentration in 3 mL reaction mixture? and if there is any donor is present in 3 mL mixture how to select the concentration of...

07 August 2016 8,136 1 View

How does the surface roughness on hydrophilic materials change to superhydrophilic surfaces?

Nano/micro roughness causes an increase or decrease in the the contact angle depending on the nature of solid substrates. Trapped air causes an increase in the contact angle for hydrophobic...

29 December 2014 5,341 6 View

Why does the ambipolar diffusion happen in organo metal halide perovskites?

Perovskites are becoming popular materials for solar cells.This is mainly due to the optimal band gap and property of ambipolar diffusion of electrons and holes.

21 December 2014 6,695 4 View

Why are band tails exponential nature in amorphous materials?

In crystalline materials the conduction band (C.B) and valence band (V.B) are parabolic nature and has a sharp mobility edges. In amorphous case the sharp edge disappears because of defects but it...

28 March 2014 6,152 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View