Anyone has an experience extracting proteins from rectal swab samples?

I am working with rectal swab samples in the field of colorectal cancer research. Does anyone have experience (or can point towards any relevant literature) of specifically working with proteins...

02 February 2021 3,622 3 View

How do you check the occupied d-orbitals in VASP?

I am running VASP calculations involving transition metal atoms in which there are multiple unpaired electrons in the d-orbitals but I am not sure how to check the individual d-orbitals (ex:...

24 August 2020 1,947 2 View

Can you check spin multiplicity on individual atoms in VASP?

I am doing VASP calculations with a bulk triclinic lattice system involving transition metals Cr and V. I know that you can check the spin for the entire system with the OSZICAR file from VASP but...

16 July 2020 9,052 3 View

How to make a scatter plot for many variables and 2 groups in one graph?

I have 2 groups and have 7 variables. I would like to make a stacked scatter graph to present it all in one graph. Is it possible and how?

13 July 2020 2,145 3 View

Beta value calculation of absolute intracellular calcium concentrations?

Hi all, I am doing calcium imaging experiments in N2a cells and wanted to calculate the absolute calcium concentrations. Can someone help me out in figuring out how to calculate the fluorescence...

24 March 2020 7,974 1 View

Workfunction from LOCPOT?

Does anyone know how to derive the work function from the LOCPOT file from vasp?

24 March 2020 851 5 View

I have 2 groups to compare by removing the effects of age and the data is not normal. How do I do so in SPSS?

The 2 groups are parents and their children. The data is not normal. Would like the comparison without the effects of age.

21 February 2020 7,714 4 View

Hi, I have child-mother data on a quantitative trait and need to know how to calculate their heritability?

I have done a spearman correlation as the data is not normally distributed and now would want heritability index...am I correct in looking at heritability with this data? If yes, how do I go about?

14 February 2020 9,160 3 View

Experimental method for determination of C-H (homolytic) BDE in organic compounds?

Hello Everyone, We want to measure the C-H (homolytic) bond dissociation energy of some organic compounds. Any practically feasible tool in the common analytical laboratory that can give us the...

13 February 2020 7,588 2 View

How to decide on which MDR strain (GN bacterium) to buy from ATCC?

I want to purchase well characterized MDR strains of a Gram-negative bacterium from ATCC to look at modes of resistance. I am also going through literature to see if people have purchased a...

05 June 2019 7,721 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View