Hi, I am looking to study transcriptional burst and RNA Pol II pausing at the promoter of a gene. What are the best methods to study such events at a locus? Most of the papers I saw focus on high throughput techniques.
TIA!
My research objective is to find the impact of CSR on financial performance of the companies. How to measure the CSR as independent variable?
14 July 2024 2,846 5 View
I purified a protein containing His tag via Ni-NTA chromatography wherein the protein was eluted in elution buffer containing 500mM imidazole, 50 mM Tris, 500mM Nacl. Since my next step of...
31 March 2024 1,298 2 View
which journal is best for a pathology/gasteroenterology case report to be published with no publication fee?
05 March 2024 7,427 2 View
In Indian context i am trying to verify the financial performance in relation to CG and CSR. Hence for empirical analysis this is required.
19 January 2024 5,593 6 View
I want to know whether people enjoy listening to music generated through AI models and machine learning and whether people prefer this? Is there a difference between how legal professionals,...
04 December 2023 9,197 1 View
how can i add all my articles and journals in research gate including those published in research gate?
22 November 2023 5,926 3 View
I need clarity regarding the following parameters 1) Condenser Temperature ( Should I go for -50 degrees or -80 degrees) 2) Vacuum pressure to be applied
11 August 2023 8,761 1 View
In order to assess the telomere length in human blood samples by qpcr, what is the best standard reference one can use for drawing the standard curve? I came across papers which use a pool of...
03 March 2023 2,324 7 View
I am inducing ischemia by OGD in vitro. If I want to estimate mitochondrial membrane potential or look at ROS levels by means of fluorescent dyes, can I fix the cells and then stain for these? I...
17 January 2023 2,256 4 View
In Psychological Assessment and Testing, how the scale, test, battery, and inventory are similar and different from each other?
09 January 2023 3,762 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,537 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,688 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,619 1 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,561 0 View
how to get links for copyrights for papers?
06 August 2024 7,409 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 806 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,365 3 View