Rajivgandhi Sundaram

2 Questions 13 Answers 0 Followers

Questions related from Rajivgandhi Sundaram

What's the problem with my GST-Fusion protein expression?

I have cloned a gene with N terminally fused GST in pGEX6P1. II expressed it in BL21(DE3) pLysS cells induced at 0.6 OD 600 with 1mM IPTG, 16 degree celcius overnight in 4 ml of 2YT medium...

11 August 2014 9,532 4 View

Why is a GST-fusion protein not expressed?

I have cloned a gene in pGEX5X1 with N-terminal GST, C-terminal Flag tag ( total length of protein is 750 amino acid excluding tags ) that is fully unstructured in c-terminal half, and comprising...

24 June 2014 5,052 13 View