3 Questions 23 Answers 0 Followers
Questions related from Rajivgandhi Sundaram
Dear colleagues, A trimeric protein of 90 KDa in (25 mM Tris-HCl (pH 7.5), 100 mM NaCl, and 2 mM DTT) formed crystals at 18 degree in overnight with conditions having 4 or 8 % tacsimate pH 5.0, 12...
13 September 2016 5,096 10 View
I have cloned a gene with N terminally fused GST in pGEX6P1. II expressed it in BL21(DE3) pLysS cells induced at 0.6 OD 600 with 1mM IPTG, 16 degree celcius overnight in 4 ml of 2YT medium...
11 August 2014 9,582 4 View
I have cloned a gene in pGEX5X1 with N-terminal GST, C-terminal Flag tag ( total length of protein is 750 amino acid excluding tags ) that is fully unstructured in c-terminal half, and comprising...
24 June 2014 5,116 13 View
