Beta value calculation of absolute intracellular calcium concentrations?

Hi all, I am doing calcium imaging experiments in N2a cells and wanted to calculate the absolute calcium concentrations. Can someone help me out in figuring out how to calculate the fluorescence...

24 March 2020 7,974 1 View

What is the significance of 'dark spectral response'?

Can anyone explain me the significance of ''dark spectral response'' or " Dark EQE" of solar cell and how it will help to improve the efficiency of solar cell?

17 April 2019 7,021 6 View

What can I find out by performing DSC on a polymer sample? Also how would I interpret a curve that I obtained?

Metallurgical engineer who has never performed DSC and now I am working with polymers. Am able to do the test on the equipment however I am still facing troubles with interpreting the curve that I...

06 March 2018 7,660 4 View

Which polymer can be in rich in surface when a mixture of two hydrophobic polymers coated on substrate?

"A" is the polymer having low molecular weight and low surface energy. "B" is the polymer with high molecular weight and high surface energy. In a mixture "B" is slightly large concentration than...

31 October 2016 1,742 2 View

How do I select the best primer which does not produce any non specific bands from those I have designed?

Hi! I am currently designing primers for the human UGT1A1 gene using the Primer3 software. However, when I BLAST the primers against the Human RefSeq database, I get significant matches with other...

10 October 2016 7,172 3 View

How to perform enzyme activity assays?

If i want to perform any enzyme activity how to select the substrate concentration in 3 mL reaction mixture? and if there is any donor is present in 3 mL mixture how to select the concentration of...

07 August 2016 8,136 1 View

How to prepare origami competent cells?

Should i need to add antiboitcs ? if so what is the concentrations?

19 July 2016 5,161 2 View

How does the surface roughness on hydrophilic materials change to superhydrophilic surfaces?

Nano/micro roughness causes an increase or decrease in the the contact angle depending on the nature of solid substrates. Trapped air causes an increase in the contact angle for hydrophobic...

29 December 2014 5,341 6 View

Why does the ambipolar diffusion happen in organo metal halide perovskites?

Perovskites are becoming popular materials for solar cells.This is mainly due to the optimal band gap and property of ambipolar diffusion of electrons and holes.

21 December 2014 6,695 4 View

FTIR spectroscopy analysis for membrane fouling?

When a fouled membrane like Nafion was analyzed for FTIR analysis, before mesurment should we remove the flock (layer of bacterial biofilm) on the membrane or not? For the assessment of membrane...

06 July 2014 1,560 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

How to determine a priori sample size for t-test with unequal means?

I need to conduct a a priori sample size determinations for t-test with unequal means, but I don't know the sizes of the groups nor the ratio of both groups... Can I do a sample size determination...

24 February 2021 8,289 3 View