My r-protein (in e.coli) is only solublized in urea and I want touse it in a downsteam immunization procedure, dentaured protein is not convenient for production of Ab, how to solve this problem ?
Omar Gonzalez-Ortega
Need to run a folding procedure first, you need to get rid of urea as slow as possible. You can use dialysis, dilutions, on-column folding, etc. No matter the selected method folding is tricky and depends on several variables such as your protein and the solvent you are using for folding.
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Vicente Mendoza Reyes
After having done the refolding step (and getting rid of urea) as described above by @OmarOrtega, you need to think about what adjuvant(s) and/or immunopotentiator(s) to include in your immunization in order to optimize the immune response you want. HTH.
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