Haitham Tartor

12 Questions 24 Answers 0 Followers

Questions related from Haitham Tartor

Normality and equality of varience testing?

Hi guys, I have an experiemnt where three groups of fish were used, two of them were treated with two doses of virus, while the thrid is a control group. I want to compare the means of gene...

23 July 2019 992 4 View

How to remove sarkosyl before mice immunization?

is it necessary to remove the sarkosyl- my r-protein is solubilized in sarkosyl- before mice immunization?

16 March 2017 1,247 2 View

How to prepare my proein for mice immunization?

My r-protein (in e.coli) is only solublized in urea and I want touse it in a downsteam immunization procedure, dentaured protein is not convenient for production of Ab, how to solve this problem ?

16 March 2017 8,084 2 View

Does treatment of r-protein with DTT impacts the mAB production?

I want to know if any of you has an idea about how much does the using of a DTT treated protein (as immunogen in mice) affects the mAb production process?

02 March 2017 9,420 4 View

Can I use precipitated or aggregated r-protein for immunization?

I will purify my r-protein (produced in E.coli) and cleave the MBP and His-tag off it and I have heard about the inclination of the r-protein to aggregate/precipitate when the tag cleavage takes...

01 March 2017 5,349 1 View

Urea as protein denaturant?

Do you know If the urea is quite more efficient than SDS as a protein denaturant? I always experience a smear in my SDS-PAGE and I thought Urea may help me in getting rid of this smear.

25 November 2015 2,795 13 View

Scattering and autofluorescnce of hek 293 suspended cells?

Ask: I want to share the results of my experiment to confirm the transfection rate of hek 293 cells (suspended not adherent) with a plasmid that reports with GFP, 2 days and 5 days after...

27 October 2015 2,354 4 View

How do I dilute my serum for affinity purification ?

Hi guys, I am going to conduct an affinity purification experiment using a synthetic peptide coupled to an agarose matrix to pick up the specific Abs from the pAb fro my rabbit serum, my question...

21 October 2015 6,508 2 View

Any advice for skin leukocytes isolation from fish?

problem of mucus and epithelial cells when isolating skin leukocytes?

07 October 2015 7,653 0 View

Usually with IHC protocol, I wash before and after H2O2 with TBS buffer, recently I found some protocols use dH2O instead of buffer ?

Usually with IHC protocol, I wash before and after H2O2 with TBS buffer, recently I found some protocols use dH2O instead of the buffer, which one is better?

11 September 2015 8,776 3 View

Is compensation needed for PI and FITC stained cells (FITC against IgM and PI for cell viability testing)?

If yes, what control I should prepare?

27 August 2015 2,064 3 View

Can the protein concentration increase after solubilization with triton X-100?

My r-protein was 1.1 mg/ml and gives smear during SDS I decided to use Triton x-100 to solubilize my protein, but the problem is that the conc. increased from 1.1 to 4 mg/ml is that logic

19 August 2015 7,274 7 View