21 Questions 52 Answers 0 Followers
Questions related from Haitham Tartor
Hi guys, I have an experiemnt where three groups of fish were used, two of them were treated with two doses of virus, while the thrid is a control group. I want to compare the means of gene...
23 July 2019 1,132 4 View
is it necessary to remove the sarkosyl- my r-protein is solubilized in sarkosyl- before mice immunization?
16 March 2017 1,368 2 View
My r-protein (in e.coli) is only solublized in urea and I want touse it in a downsteam immunization procedure, dentaured protein is not convenient for production of Ab, how to solve this problem ?
16 March 2017 8,208 2 View
I want to know if any of you has an idea about how much does the using of a DTT treated protein (as immunogen in mice) affects the mAb production process?
02 March 2017 10,102 4 View
If my protein is tagged with both his and MBP tag and it has a Tev site as well just between the MPB and the target protein, which way is better to remove the tag after Tev treatment (Amylose...
02 March 2017 2,440 4 View
I will purify my r-protein (produced in E.coli) and cleave the MBP and His-tag off it and I have heard about the inclination of the r-protein to aggregate/precipitate when the tag cleavage takes...
01 March 2017 6,002 1 View
I have purified my plasmid from bacterial culture -prepared from different positive colonies in colony PCR- using Iso-probanol precipitation method, and I ran 2500 ng of the purified plasmid on...
26 January 2017 9,460 7 View
I am working on a C5-mAb production project in fish and most of the clones I got after sub-clonning are of IgM isotype Is there any method that could orient the mice to give IgG instead o IgM?
31 March 2016 4,658 5 View
Why it is tricky to sequence the recombinant immunoglobulins with MS ?
28 December 2015 5,880 2 View
Do you know If the urea is quite more efficient than SDS as a protein denaturant? I always experience a smear in my SDS-PAGE and I thought Urea may help me in getting rid of this smear.
25 November 2015 2,918 13 View
Ask: I want to share the results of my experiment to confirm the transfection rate of hek 293 cells (suspended not adherent) with a plasmid that reports with GFP, 2 days and 5 days after...
27 October 2015 2,481 4 View
I have a problem with the scattering gate of the hek 293 suspended cells in flowcytometry, shall I use the log or linear option? what the range to expect my cells appeared in? Do the hek cells...
27 October 2015 8,987 3 View
Hi guys, I am going to conduct an affinity purification experiment using a synthetic peptide coupled to an agarose matrix to pick up the specific Abs from the pAb fro my rabbit serum, my question...
21 October 2015 6,604 2 View
Dear there, We are trying to characterize our novel Ab against salmon TNF-a (FITC-labelled) using flow cytometer and we have some problems, I hope you may help me with. 1- Sometimes we get more...
08 October 2015 6,782 2 View
problem of mucus and epithelial cells when isolating skin leukocytes?
07 October 2015 7,739 0 View
Usually with IHC protocol, I wash before and after H2O2 with TBS buffer, recently I found some protocols use dH2O instead of the buffer, which one is better?
11 September 2015 8,884 3 View
I want to collect leukocytes from fish skin, can anybody recommends me a protocol, I know that using a dispase could be helpful but I need more details. Thanks :)
08 September 2015 1,231 2 View
If yes, what control I should prepare?
27 August 2015 2,141 3 View
After preparation and staining of my IHC slides, I found some air bubbles under the cover slip and I want to get rid of that so I decided to incubate my slides in Xylene for 3 days, I am afraid...
20 August 2015 8,712 21 View
My r-protein was 1.1 mg/ml and gives smear during SDS I decided to use Triton x-100 to solubilize my protein, but the problem is that the conc. increased from 1.1 to 4 mg/ml is that logic
19 August 2015 7,375 7 View
what does it mean If I got 2 peaks in nanodrop graph for protein conc. I added triton X to the r protein to get rid of some annoyed smear
19 August 2015 5,264 3 View
