16 Questions 31 Answers 0 Followers
Questions related from Haitham Tartor
Hi guys, I have an experiemnt where three groups of fish were used, two of them were treated with two doses of virus, while the thrid is a control group. I want to compare the means of gene...
23 July 2019 1,011 4 View
is it necessary to remove the sarkosyl- my r-protein is solubilized in sarkosyl- before mice immunization?
16 March 2017 1,265 2 View
My r-protein (in e.coli) is only solublized in urea and I want touse it in a downsteam immunization procedure, dentaured protein is not convenient for production of Ab, how to solve this problem ?
16 March 2017 8,103 2 View
I want to know if any of you has an idea about how much does the using of a DTT treated protein (as immunogen in mice) affects the mAb production process?
02 March 2017 9,460 4 View
I will purify my r-protein (produced in E.coli) and cleave the MBP and His-tag off it and I have heard about the inclination of the r-protein to aggregate/precipitate when the tag cleavage takes...
01 March 2017 5,385 1 View
I am working on a C5-mAb production project in fish and most of the clones I got after sub-clonning are of IgM isotype Is there any method that could orient the mice to give IgG instead o IgM?
31 March 2016 4,580 5 View
Why it is tricky to sequence the recombinant immunoglobulins with MS ?
28 December 2015 5,794 2 View
Do you know If the urea is quite more efficient than SDS as a protein denaturant? I always experience a smear in my SDS-PAGE and I thought Urea may help me in getting rid of this smear.
25 November 2015 2,812 13 View
Ask: I want to share the results of my experiment to confirm the transfection rate of hek 293 cells (suspended not adherent) with a plasmid that reports with GFP, 2 days and 5 days after...
27 October 2015 2,373 4 View
I have a problem with the scattering gate of the hek 293 suspended cells in flowcytometry, shall I use the log or linear option? what the range to expect my cells appeared in? Do the hek cells...
27 October 2015 8,897 3 View
Hi guys, I am going to conduct an affinity purification experiment using a synthetic peptide coupled to an agarose matrix to pick up the specific Abs from the pAb fro my rabbit serum, my question...
21 October 2015 6,516 2 View
Dear there, We are trying to characterize our novel Ab against salmon TNF-a (FITC-labelled) using flow cytometer and we have some problems, I hope you may help me with. 1- Sometimes we get more...
08 October 2015 6,702 2 View
problem of mucus and epithelial cells when isolating skin leukocytes?
07 October 2015 7,660 0 View
Usually with IHC protocol, I wash before and after H2O2 with TBS buffer, recently I found some protocols use dH2O instead of the buffer, which one is better?
11 September 2015 8,788 3 View
If yes, what control I should prepare?
27 August 2015 2,073 3 View
My r-protein was 1.1 mg/ml and gives smear during SDS I decided to use Triton x-100 to solubilize my protein, but the problem is that the conc. increased from 1.1 to 4 mg/ml is that logic
19 August 2015 7,284 7 View
