How to prepare mouse brain tissue for co-immunoprecipitation?

10 October 2017 1 10K Report

What buffer is best used in a co-IP protocol when using mouse brain tissue? What protocols would you recommend to prepare the tissue for co-IP (e.g. abcam, thermo-fisher, etc)?

Mirsada Causevic

It all depends on sub-cellular localisation(s) of your proteins of interest. It is best to look up articles that describe your proteins of interest and to prepare your own buffers - because you know exactly what you have put into them (the buffers).

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