What dilution factor to use for TLR4 antibody in immunoprecipitation (lysed brain tissue)?

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Michael Davis

This will be dependent on your sample, antibody, and incubation time and will be something you'll have to determine empirically. A good place to start would be 0.5, 1.0, and 2.0 ug/mL antibody for 1 mg/mL protein lysate. Conduct your IP's. Pull your IP supernatants off after IP and analyze them by western to see wether or not you've cleared your lysate. Compare the signal to what is on the beads as well as your pre IP lysate. Find the concentration of antibody that clears your lysate and then double it to be safe. Good luck.

Okan Akar

Hello Ilse Flores,

I would like to know which ab you are using. Dilution factor must be written on Data Sheet and maybe if some of us used same brand of ab can suggest.

Good day.