Ilse Flores

4 Questions 1 Answers 0 Followers

Questions related from Ilse Flores

What is the best way to collect weekly blood samples from mice that are being exercised?

I plan to carry out a study where I run mice on a treadmill for 6 weeks. I would like to have blood samples from before they exercise and at weekly timepoints. Briefly, we want to look at...

02 February 2020 358 1 View

What are the best TLR4 & MyD88 antibodies one could use in co-immuniprecipitation (lysed brain tissue)?

We will be lysing mouse brain tissue for the co-IP of TLR4 and MyD88, followed by a western blot.

10 October 2017 4,525 2 View

What dilution factor to use for TLR4 antibody in immunoprecipitation (lysed brain tissue)?

What antibody dilution factor is most effective in the IP of TLR4? We will be using lysed mouse brain tissue.

10 October 2017 8,648 2 View

How to prepare mouse brain tissue for co-immunoprecipitation?

What buffer is best used in a co-IP protocol when using mouse brain tissue? What protocols would you recommend to prepare the tissue for co-IP (e.g. abcam, thermo-fisher, etc)?

10 October 2017 9,724 1 View