I plan to carry out a study where I run mice on a treadmill for 6 weeks. I would like to have blood samples from before they exercise and at weekly timepoints. Briefly, we want to look at peripheral markers of inflammation.
I was considering either the tail vein method, cheek, or retro-orbital. Which would interfere the least with their ability to run, provide the least amount of stress, and allow for weekly collections?
How much blood per sample? According to my lab animal veterinarian girlfriend, 300 µl obtained on a weekly basis via the jugular vein is about the most you can ask for and not drastically affect physical performance. It would be nice if you could transfuse blood after taking your weekly samples.
The tail vein method is not good for relatively large samples.
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