Can anyone help with brain tissue protein solubilization in Lammelli buffer after precipitation?

I had homogenized protein in RIPA buffer but was facing problem in running SDS-PAGE gel. So I precipitated protein with 100% TCA , washed with Acetone and then tried to resolubilize protein in 2X...

03 April 2015 5,589 3 View

Query regarding small size protein transfer on Nitrocellulose membrane ( pore size- 0.45u).

My protein is of molecular weight below 20 kDa. I transferred it for 30 min at 90V but could not see distinct bands i.e. smear type on Ponceau staining.15% gel was used and was run half of the...

11 December 2013 9,284 3 View

Related to western blot protein bands on X-ray film?

Transfer of proteins on nitrocellulose membrane is fine. I am following the standard protocol but I am not getting band of b-actin on X-ray film so can anyone suggest what could be the problem?...

08 September 2013 4,674 3 View

What is the best method of plasmid transfection in mature oligodendrocytes?

I am working on a methylating protein and would like see the expression of gene in mature oligodendrocytes. What is the best method for transient but long lasting expression?

03 April 2013 1,812 2 View

Is there any reliable bioinformatics tool for promoter sequence retrieval and primer designing for the same?

for CHip analysis

11 December 2012 6,391 7 View

do the E3 ligases which are present in stem cell stays /expresses in differentiated cell or they are regulated by specific protein expression?

topic: protein degradation by Ubiquitination for each of the protein degraded by ubiquitination , cells have specific E3 ligases. this E3 ligases are regulated by degradation of them by their own...

31 December 2011 4,109 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View