What is the best method of plasmid transfection in mature oligodendrocytes?

More Arpita Dave's questions See All

Can anyone help with brain tissue protein solubilization in Lammelli buffer after precipitation?

I had homogenized protein in RIPA buffer but was facing problem in running SDS-PAGE gel. So I precipitated protein with 100% TCA , washed with Acetone and then tried to resolubilize protein in 2X...

03 April 2015 5,670 3 View

How to prepare cuprizone pellets?

What can be used to bind the powder to prepare pellets?

07 August 2014 2,030 3 View

Query regarding small size protein transfer on Nitrocellulose membrane ( pore size- 0.45u).

My protein is of molecular weight below 20 kDa. I transferred it for 30 min at 90V but could not see distinct bands i.e. smear type on Ponceau staining.15% gel was used and was run half of the...

11 December 2013 9,400 3 View

Related to western blot protein bands on X-ray film?

Transfer of proteins on nitrocellulose membrane is fine. I am following the standard protocol but I am not getting band of b-actin on X-ray film so can anyone suggest what could be the problem?...

08 September 2013 4,757 3 View

Is there any reliable bioinformatics tool for promoter sequence retrieval and primer designing for the same?

for CHip analysis

11 December 2012 6,467 7 View

do the E3 ligases which are present in stem cell stays /expresses in differentiated cell or they are regulated by specific protein expression?

topic: protein degradation by Ubiquitination for each of the protein degraded by ubiquitination , cells have specific E3 ligases. this E3 ligases are regulated by degradation of them by their own...

31 December 2011 4,190 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View