Arpita Dave

7 Questions 13 Answers 0 Followers

Questions related from Arpita Dave

Can anyone help with brain tissue protein solubilization in Lammelli buffer after precipitation?

I had homogenized protein in RIPA buffer but was facing problem in running SDS-PAGE gel. So I precipitated protein with 100% TCA , washed with Acetone and then tried to resolubilize protein in 2X...

04 April 2015 5,649 3 View

How to prepare cuprizone pellets?

What can be used to bind the powder to prepare pellets?

08 August 2014 1,997 3 View

Query regarding small size protein transfer on Nitrocellulose membrane ( pore size- 0.45u).

My protein is of molecular weight below 20 kDa. I transferred it for 30 min at 90V but could not see distinct bands i.e. smear type on Ponceau staining.15% gel was used and was run half of the...

12 December 2013 9,368 3 View

Related to western blot protein bands on X-ray film?

Transfer of proteins on nitrocellulose membrane is fine. I am following the standard protocol but I am not getting band of b-actin on X-ray film so can anyone suggest what could be the problem?...

09 September 2013 4,734 3 View

What is the best method of plasmid transfection in mature oligodendrocytes?

I am working on a methylating protein and would like see the expression of gene in mature oligodendrocytes. What is the best method for transient but long lasting expression?

04 April 2013 1,871 2 View

Is there any reliable bioinformatics tool for promoter sequence retrieval and primer designing for the same?

for CHip analysis

12 December 2012 6,451 7 View

do the E3 ligases which are present in stem cell stays /expresses in differentiated cell or they are regulated by specific protein expression?

topic: protein degradation by Ubiquitination for each of the protein degraded by ubiquitination , cells have specific E3 ligases. this E3 ligases are regulated by degradation of them by their own...

01 January 2012 4,170 4 View