Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

How to obtain CMIP6 Global Climate Model dataset?

Hi, I am using hydrological modelling under future climate projection in conparison to cmip5 and cmip6. What are the best sourcesfor GCMs dataset for SSPs scenarios?

26 August 2020 3,703 7 View

Which source is best for vegetation map in south-east Australia and MDB?

I would like to get vegetation type details in the MDB and the Murrumbidgee River catchment area to refer it for satellite image analysis. Please also refer any relevant articles. Thank you all in...

30 March 2020 9,549 3 View

How can I extract temporal and spatial data from satellite image?

I would like to extract temporal and spatial data for LAI from a satellite image.

21 March 2020 1,470 5 View

If failure 1 occurred 5 times in a month consuming 10 hours versus failure 2 which occurred 10 times consuming 5 hours. Which should be prioritized?

Please help me to understand which failure on a production line should be prioritized when comparing failure 1 which occurred 5 times in the month and consumed 10 hours (total) versus failure 2...

03 December 2019 4,782 8 View

Particle bombardment for production of transgenic plants. Does the type of bomb have an impact on whether the gene is found or not?

Dissertation student in need of help :(

31 December 2018 2,481 0 View

What will be the trend of sound speed in higher dimensional compact star modelings?

In many articles I have seen that the sound speed within higher dimensional compact stars are increasing in trend. However, in general relativity, we tried to model compact stars not only obeyed...

19 April 2016 2,244 3 View

Can the sub fields within a field ever be truly autonomous?

If we take the field of formal education and drill down to the sub fields of mainstream and alternative education and we move a student from mainstream into alternative education, homology of...

12 March 2016 1,333 3 View

Does it matter how long my bacteria take to reach the correct OD for RNA extraction?

I am hoping to perform some RT-PCR. However my bacteria has taken longer to reach the correct OD at which I should harvest. Does this reflect in anyway the RNA which I then prep and take forward...

05 February 2013 7,401 4 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

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02 March 2021 3,060 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View