Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

How to obtain CMIP6 Global Climate Model dataset?

Hi, I am using hydrological modelling under future climate projection in conparison to cmip5 and cmip6. What are the best sourcesfor GCMs dataset for SSPs scenarios?

26 August 2020 3,703 7 View

Which source is best for vegetation map in south-east Australia and MDB?

I would like to get vegetation type details in the MDB and the Murrumbidgee River catchment area to refer it for satellite image analysis. Please also refer any relevant articles. Thank you all in...

30 March 2020 9,549 3 View

How can I extract temporal and spatial data from satellite image?

I would like to extract temporal and spatial data for LAI from a satellite image.

21 March 2020 1,470 5 View

If failure 1 occurred 5 times in a month consuming 10 hours versus failure 2 which occurred 10 times consuming 5 hours. Which should be prioritized?

Please help me to understand which failure on a production line should be prioritized when comparing failure 1 which occurred 5 times in the month and consumed 10 hours (total) versus failure 2...

03 December 2019 4,782 8 View

Particle bombardment for production of transgenic plants. Does the type of bomb have an impact on whether the gene is found or not?

Dissertation student in need of help :(

31 December 2018 2,481 0 View

What will be the trend of sound speed in higher dimensional compact star modelings?

In many articles I have seen that the sound speed within higher dimensional compact stars are increasing in trend. However, in general relativity, we tried to model compact stars not only obeyed...

19 April 2016 2,244 3 View

Can the sub fields within a field ever be truly autonomous?

If we take the field of formal education and drill down to the sub fields of mainstream and alternative education and we move a student from mainstream into alternative education, homology of...

12 March 2016 1,333 3 View

Does it matter how long my bacteria take to reach the correct OD for RNA extraction?

I am hoping to perform some RT-PCR. However my bacteria has taken longer to reach the correct OD at which I should harvest. Does this reflect in anyway the RNA which I then prep and take forward...

05 February 2013 7,401 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View