Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

How to obtain CMIP6 Global Climate Model dataset?

Hi, I am using hydrological modelling under future climate projection in conparison to cmip5 and cmip6. What are the best sourcesfor GCMs dataset for SSPs scenarios?

26 August 2020 3,703 7 View

Which source is best for vegetation map in south-east Australia and MDB?

I would like to get vegetation type details in the MDB and the Murrumbidgee River catchment area to refer it for satellite image analysis. Please also refer any relevant articles. Thank you all in...

30 March 2020 9,549 3 View

How can I extract temporal and spatial data from satellite image?

I would like to extract temporal and spatial data for LAI from a satellite image.

21 March 2020 1,470 5 View

If failure 1 occurred 5 times in a month consuming 10 hours versus failure 2 which occurred 10 times consuming 5 hours. Which should be prioritized?

Please help me to understand which failure on a production line should be prioritized when comparing failure 1 which occurred 5 times in the month and consumed 10 hours (total) versus failure 2...

03 December 2019 4,782 8 View

Particle bombardment for production of transgenic plants. Does the type of bomb have an impact on whether the gene is found or not?

Dissertation student in need of help :(

31 December 2018 2,481 0 View

What will be the trend of sound speed in higher dimensional compact star modelings?

In many articles I have seen that the sound speed within higher dimensional compact stars are increasing in trend. However, in general relativity, we tried to model compact stars not only obeyed...

19 April 2016 2,244 3 View

Can the sub fields within a field ever be truly autonomous?

If we take the field of formal education and drill down to the sub fields of mainstream and alternative education and we move a student from mainstream into alternative education, homology of...

12 March 2016 1,333 3 View

Does it matter how long my bacteria take to reach the correct OD for RNA extraction?

I am hoping to perform some RT-PCR. However my bacteria has taken longer to reach the correct OD at which I should harvest. Does this reflect in anyway the RNA which I then prep and take forward...

05 February 2013 7,401 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View