I have a DIG Northern Blotting starter kit. Now I want to prepare probe for selected miRNAs.
Hi Friends, I have to isolate the complete coding part of the selected gene which size is 2.5kb contain 12 Exons. Particularly Exon 1 (Starting bases) has GC content high as >70% (high energy...
04 May 2018 3,686 2 View
HI, Currently I am using cuffdiff for DE gene analysis in 2 condition (sample and treatment). Is there any package support for my work better!
06 July 2017 660 26 View
Sequencing has done on Illumina Platform and Raw reads in FASTQ file format. How to check the read quality?
03 April 2016 2,279 5 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View
I have this problem with my western blot for the ABCG2 antibody on nitrocellulose membrane. Help please
02 July 2024 8,715 2 View