Ellango Ramasamy

4 Questions 14 Answers 0 Followers

Questions related from Ellango Ramasamy

Facing difficulty in isolation of complete CDS of selected gene?

Hi Friends, I have to isolate the complete coding part of the selected gene which size is 2.5kb contain 12 Exons. Particularly Exon 1 (Starting bases) has GC content high as >70% (high energy...

05 May 2018 3,655 2 View

Which package is better for calculate DE genes?

HI, Currently I am using cuffdiff for DE gene analysis in 2 condition (sample and treatment). Is there any package support for my work better!

07 July 2017 618 26 View

We have obtained the NGS small RNA sequencing Raw reads (Outsourcing) How to check the sequence integrity?

Sequencing has done on Illumina Platform and Raw reads in FASTQ file format. How to check the read quality?

04 April 2016 2,239 5 View

How to prepare a Micro RNA probe (manual) for Northern blotting?

I have a DIG Northern Blotting starter kit. Now I want to prepare probe for selected miRNAs.

04 April 2016 7,310 0 View