Grow the bacteria biofilm by immersing the biofilm support/surface material in the growth medium inoculated (say 10% v/v) with pure cultures of test bacteria for at least 12 hrs. transfer support materials with adhered seed cells to fresh medium for the biofilm to grow and mature. You can perform cell counts microscopically using crystal violet staining technique or using spectrophotometry for optical density determination. Go through these documents for details.