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Questions related from Gerry/Gerre A Quinn
I was wondering what the difference between fresh air and indoor air is and why is it supposed to be better for your health. I think fresher air has a different microbiome, but is this the reason...
23 June 2025 6,553 3 View
Looking for text of this article Gastroliths in the stomachs of common pheasant and grey partridge P. Hell, J. Slamecka, T. Bialas Published 1995 Biology, Environmental Science
11 February 2025 3,188 0 View
Recently reading about viral biofilms, I wondered if it was possible for lipid nanoparticles to merge with the extracellular matrix and deliver their cargo?
04 November 2024 5,330 2 View
The COVID-19 mRNA vaccine was designed to bypass the innate immune system so that there would not be an overreaction from the immune system. Could there be any possible negative effects from this?
06 October 2024 4,001 4 View
I was looking for the most recent antibiotic to be approved for clinical use that has a significantly different structure from previous antibiotics. Preferrably sourced from the natural...
05 October 2023 3,357 3 View
If you can change your lifestyle/behaviour, how soon can these changes be detected in the epigenome? How long (estimate) would this change be detected in the majority of cells? Can this change...
22 July 2023 3,976 1 View
I am adding different compounds to agar plate wells which I overlay with a shallow layer of Sabouraud dextrose agar on which i spread Bakers yeast. It takes around 24h to see zones of inhibition...
29 July 2022 1,503 0 View
I am looking for the WGS of a variety of Chickens (Gallus gallus) such as Rode Island Red or White Leghorn. I realize there might not be a great diversity between these breeds but I would like to...
22 April 2022 5,273 1 View
I am trying to identify some small molecules in a bacterial supernatant extract. I would like to remove proteins to make mass spectrometry easier. I have tried protein crash with ACN but the...
04 March 2021 4,862 4 View
WGS of Streptomyces sp. myrophorea revealed 31 gene matches for daunorubicin/doxorubicin resistance genes. I thought this might also be an indicator of production but antiSMASH did not show...
12 April 2020 5,695 0 View
Can anyone tell me the largest number of proteins directly extracted from soil ? I am writing a paper about soil protein extraction and wanted to know what sort of yields other researchers were...
31 January 2020 9,277 2 View
How do I order my data for analysis in Prism by Mann Whitney U test for nonparametric data ? I have two conditions to extract proteins in replicates of 3. There are many blank results in the...
14 August 2019 4,187 2 View
I am interested in the stages of Streptomyces life cycle involving breakdown of old mycelia and their possible reuse. Is it possible to direct me to some focussed papers on this subject ?
17 June 2019 6,154 0 View
We are looking for a Candicidin D standard for HPLC and mass spectrometry. It is no longer made by the company listed on PubChem (alpha chemistry). Does anybody have any other suggestions?
16 January 2019 2,675 3 View
I performed some comparitive proteomics between planktonic and biofilm membrane proteomes using tandem mass tags. The mass spectrometry generated HCID and CID data . I now need to identify all...
14 August 2018 6,164 3 View
I am conducting a metagenomic analysis of alkaline soil and I wondered if there were any specialised methods of DNA extraction given the alkaline nature of the soil.
04 August 2018 2,271 2 View
I am assessing some protein extraction techniques and find that nitrogen cycle regulation proteins seem to cluster ! or are being depleted by some of the other techniques I am using. Are there...
02 August 2018 7,471 0 View
Given the discovery of many antimicrobial defense compounds on the skin which protect against infection , I was wondering if there were any compounds that protect the skin against sunlight in...
24 June 2018 7,932 6 View
I had previously made a series of (modified SFM) agars at high alkaline pH 8----->13.6. I did not measure the pH of the resulting solidified agar because it just seemed a bit awkward and I...
08 March 2018 1,338 3 View
I was reading about the increase in detection of PTM phosphopeptides by the incorportaion of off-line high-pH reverse-phase fractionation and I wondered if the digestion of a proteome (not the...
16 February 2018 6,002 4 View
Many proteins have multiple GO terms for biological function. I want to take these other functions into account when analysing functional changes between two stages of bacterial cell growth. The...
14 December 2017 2,034 1 View
I was wondering if the pH of an environment influences the development of amyloid proteins ? Cellular control of pH is performed by porters/ antiporters. I have searched for these in human...
08 November 2017 1,401 4 View
I have been using activated carbon columns to clean up protein samples. Protein recovery has been very low. When I use a large amount of protein i.e. 5mg the recovery is only 0.5mg . I was...
11 October 2017 5,694 1 View
I am testing the inhibitory potential of some environmental Streptomyces. Some of the zones of inhibition are not immediately apparent and you have to tilt the plates against the light to see the...
24 August 2017 4,246 5 View
After repeatedly sub-culturing some of my strains of Streptomycetes, I find that their antimicrobial potential is diminishing. Are there some good methods for re-awakening their antimicrobial...
22 May 2017 9,500 6 View
I have a protein solution with 4% SDS. I want to remove the SDS for later downstream analysis. I know a few techniques for protein precipitation , actone/ethanol, choloform/methanol, TCA/acetone,...
31 March 2017 8,424 6 View
I am interested in the mechanisms of pH control in Streptomyces through Na+/H+ antiporters. Are there any good papers describing the control of these antiporters ?
21 March 2017 8,547 2 View
I was looking for antibiotic genes/clusters in a recently sequenced strain of Streptomyces. I was wondering if the presence of 4'-demethylrebeccamycin gene suggested rebeccamycin synthesis ?
19 March 2017 4,144 0 View
I was wondering if there are any relatively inexpensive compounds that mimic the human skin ? I was thinking back to my days experimenting with Schistosoma parasites and we used skin surface...
18 February 2017 2,724 2 View
I am trying to clean up some colour from bacterial protein. Graphite columns work quite well but they only hold 100ug of protein. Are there any suppliers of higher capacity columns in europe (i...
10 February 2017 6,249 4 View
I was wondering if phosphorylation was necessary for amyloid fibre formation ? I have read a lot about eukaryotic phosphorylation in the case of alzheimers etc . I wondered if it was generally...
17 December 2016 749 3 View
I was looking for a method to detect and measure oxytetracycline by rpHPLC. I have read many methods that use C8 or C18 columns in combination with formic, oxalic or TFA acids and acetonitrile...
31 October 2016 1,314 4 View
I am seeing a lot of background staining on pIMAGO kit for phosphorylated proteins on membrane. I am using PVDF membrane . All reagents were freshly prepared with milliQ water etc. Does anybody...
23 October 2016 9,867 2 View
I have one bulk soil sample which i will extract by two different methods. Both methods have around 6-7 steps before the purified protein is ready for mass spectrometery identification of the...
18 July 2016 378 5 View
I want to measure protein phosphorylation in bacterial cell extracts. we have a protocol using antibodies but it is quite time consuming. I have read of a method which uses pIMAGO and I...
15 June 2016 3,274 2 View
In previous research I tested the effectiveness of biosurfactants on the removal of microbial biofilms. Having moved more into proteomics I have not kept abreast of current developments in...
12 February 2016 2,656 3 View
I am trying to sequence amyloid peptides (in this case chaplins/rodlins from streptomyces) by mass spectrometry but this is hampered by lack of trypsin cleavage sites. We have also tried...
06 January 2016 380 1 View
I am trying to identify proteins from soil using mass spectrometry with a soil protein database. Are there any good pipelines out there that can take protein fasta files, assemble/join them all...
08 October 2015 4,768 0 View
I have been trying to identify proteins extracted from soil using tandem mass spectrometry. I am analysing the tryptic digest ms/ms data using different databases such as NCBI nr, swissprot, NCBI...
05 May 2015 9,052 10 View
I would like to know researchers' experiences in quantifying amyloid proteins. More specifically those found in environmental samples like chaplins/hydrophobins /rodlins/environmental biofilm...
24 November 2014 9,096 5 View
I am looking at the proteome of soils with a high humic content, however the analysis is always complicated by humic acids, which co-extract with almost every purification procedure. Therefore I...
28 May 2014 4,767 12 View
