Is anyone tried to run Cytochrome C tryptic digest on UPLC?

We have ACQUITY UPLC Peptide BEH C18, 130Ao,1.7um, 2.1*150mm for Peptide mapping. The mobile phase which were tried 1) A: 0.045% H2O, B:.045%ACN 2)A: 0.045% H2O, B:.045%ACN Various gradient were...

05 June 2019 2,265 2 View

Can a single cyclic peptide shows two peaks in HPLC?

Single cyclic peptide in LC having two peaks.

04 May 2019 9,318 7 View

How DTNB (Eliman's Reagent) can be used to detect free thiol in protein ?

I am trying to detect free thiol in protein. But the confirmation of free thiol with DTNB should be done visibly (by colour change).I have already tested this with benzyl mercaptan and cystiene...

03 April 2019 1,353 4 View

In column chromatography alumina can be used in place of silica as a stationary phase if the compound are sticking to silica ?

31 December 2016 6,347 7 View

How Triisopropylsilane helps in cleaving peptide from resin ?

I am using Cocktail with 10% Tips, 5% phenol, 5% H2O, 80% TFA , for cleaving peptide from resin. The peptides have cysteine.

31 December 2016 7,047 1 View

How to do desalting in peptide reactions to get good mass spectrum by LC-MS ?

I am trying reactions of peptide with organic molecule in phosphate buffer (pH 7.0) .But while monitoring the reaction by LCMS due to higher salt concentration ,I am not getting the Mass...

08 September 2016 2,951 3 View

How to get the peptide containing cysteine without oxidation?

I have synthesized few peptides containing cysteine, but in this case the peptide have sulfur in oxidized form (sulfoxide not disulfide).I am using solid phase peptide synthesizer.

07 August 2016 8,806 11 View

I need a protein which is having single cysteine(solvent exposed ,Mol. wt. less than 30 Kda)?

07 August 2016 4,444 3 View

Can I get the proteins which has single cysteine with molecular weight less than 30kilodalton?

Can I get the proteins which has single cysteine with molecular weight less than 30kilodalton?

04 May 2016 5,182 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View