Is anyone tried to run Cytochrome C tryptic digest on UPLC?

We have ACQUITY UPLC Peptide BEH C18, 130Ao,1.7um, 2.1*150mm for Peptide mapping. The mobile phase which were tried 1) A: 0.045% H2O, B:.045%ACN 2)A: 0.045% H2O, B:.045%ACN Various gradient were...

05 June 2019 2,265 2 View

Can a single cyclic peptide shows two peaks in HPLC?

Single cyclic peptide in LC having two peaks.

04 May 2019 9,318 7 View

How DTNB (Eliman's Reagent) can be used to detect free thiol in protein ?

I am trying to detect free thiol in protein. But the confirmation of free thiol with DTNB should be done visibly (by colour change).I have already tested this with benzyl mercaptan and cystiene...

03 April 2019 1,353 4 View

In column chromatography alumina can be used in place of silica as a stationary phase if the compound are sticking to silica ?

31 December 2016 6,347 7 View

How to prepare a best sample for LCMS analyis?

10 November 2016 8,486 1 View

How to do desalting in peptide reactions to get good mass spectrum by LC-MS ?

I am trying reactions of peptide with organic molecule in phosphate buffer (pH 7.0) .But while monitoring the reaction by LCMS due to higher salt concentration ,I am not getting the Mass...

08 September 2016 2,951 3 View

How to get the peptide containing cysteine without oxidation?

I have synthesized few peptides containing cysteine, but in this case the peptide have sulfur in oxidized form (sulfoxide not disulfide).I am using solid phase peptide synthesizer.

07 August 2016 8,806 11 View

I need a protein which is having single cysteine(solvent exposed ,Mol. wt. less than 30 Kda)?

07 August 2016 4,444 3 View

Can I get the proteins which has single cysteine with molecular weight less than 30kilodalton?

Can I get the proteins which has single cysteine with molecular weight less than 30kilodalton?

04 May 2016 5,182 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View