Is anyone tried to run Cytochrome C tryptic digest on UPLC?

We have ACQUITY UPLC Peptide BEH C18, 130Ao,1.7um, 2.1*150mm for Peptide mapping. The mobile phase which were tried 1) A: 0.045% H2O, B:.045%ACN 2)A: 0.045% H2O, B:.045%ACN Various gradient were...

05 June 2019 2,265 2 View

Can a single cyclic peptide shows two peaks in HPLC?

Single cyclic peptide in LC having two peaks.

04 May 2019 9,318 7 View

How DTNB (Eliman's Reagent) can be used to detect free thiol in protein ?

I am trying to detect free thiol in protein. But the confirmation of free thiol with DTNB should be done visibly (by colour change).I have already tested this with benzyl mercaptan and cystiene...

03 April 2019 1,353 4 View

In column chromatography alumina can be used in place of silica as a stationary phase if the compound are sticking to silica ?

31 December 2016 6,347 7 View

How Triisopropylsilane helps in cleaving peptide from resin ?

I am using Cocktail with 10% Tips, 5% phenol, 5% H2O, 80% TFA , for cleaving peptide from resin. The peptides have cysteine.

31 December 2016 7,047 1 View

How to prepare a best sample for LCMS analyis?

10 November 2016 8,486 1 View

How to do desalting in peptide reactions to get good mass spectrum by LC-MS ?

I am trying reactions of peptide with organic molecule in phosphate buffer (pH 7.0) .But while monitoring the reaction by LCMS due to higher salt concentration ,I am not getting the Mass...

08 September 2016 2,951 3 View

How to get the peptide containing cysteine without oxidation?

I have synthesized few peptides containing cysteine, but in this case the peptide have sulfur in oxidized form (sulfoxide not disulfide).I am using solid phase peptide synthesizer.

07 August 2016 8,806 11 View

Can I get the proteins which has single cysteine with molecular weight less than 30kilodalton?

Can I get the proteins which has single cysteine with molecular weight less than 30kilodalton?

04 May 2016 5,182 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View