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Questions related from Neelesh Reddy
We have ACQUITY UPLC Peptide BEH C18, 130Ao,1.7um, 2.1*150mm for Peptide mapping. The mobile phase which were tried 1) A: 0.045% H2O, B:.045%ACN 2)A: 0.045% H2O, B:.045%ACN Various gradient were...
06 June 2019 2,308 2 View
Single cyclic peptide in LC having two peaks.
05 May 2019 9,372 7 View
I am trying to detect free thiol in protein. But the confirmation of free thiol with DTNB should be done visibly (by colour change).I have already tested this with benzyl mercaptan and cystiene...
04 April 2019 1,400 4 View
I have synthesized compound which contains amide bond, in column chromatography this compound sticking to silica? Is the use of alumina in place of silica resolve this problem?
01 January 2017 6,378 7 View
I am using Cocktail with 10% Tips, 5% phenol, 5% H2O, 80% TFA , for cleaving peptide from resin. The peptides have cysteine.
01 January 2017 7,075 1 View
If the peptides reaction were done at 30% DMSO and Phosphate buffer (0.1M, pH 7.0) with small organic molecule, so how to prepare the sample for this reaction mixture , If mobile phase for LCMS...
11 November 2016 8,513 1 View
I am trying reactions of peptide with organic molecule in phosphate buffer (pH 7.0) .But while monitoring the reaction by LCMS due to higher salt concentration ,I am not getting the Mass...
09 September 2016 2,985 3 View
I have synthesized few peptides containing cysteine, but in this case the peptide have sulfur in oxidized form (sulfoxide not disulfide).I am using solid phase peptide synthesizer.
08 August 2016 8,831 11 View
I want to modify cysteine of this protein with different electrophiles,so the cystiene should be solvent exposed.And its molecular weight should be less than 30 Kda.
08 August 2016 4,469 3 View
Can I get the proteins which has single cysteine with molecular weight less than 30kilodalton?
05 May 2016 5,213 2 View
