Hello all,
I need to prepare 5% Glutraldehyde in 0.2 M phosphate buffer. I have 25% Glutraldehyde solution in water in liquid form.
Kindly suggest me the step by step protocol to achieve this.
Please look at the following below links which may help you in your analysis:
https://www.aapd.org/globalassets/media/publications/archives/ranly-06-02.pdf
http://pathresidents.com/usapathology/em-handbook/techniques-section/glutaraldehyde.html
https://link.springer.com/chapter/10.1007%2F978-1-4419-9244-4_2
Thanks
You have to calculate the volume of 25 % glutaraldehyde first for the making of 1 % glutaraldehyde using the formula X1 V1 = X2 V2. Where, X1 is the concentration or percentage of your 25 % glutaraldehyde, V1 is the volume of 25 % glutaraldehyde (which you have to identify for 5 %), X2 is percentage of working concentration of glutaraldehyde (such as 5 %) and V1 is the volume 5 % glutaraldehyde. After the identification of the volume of 25 % glutaraldehyde, 0.2 M phosphate buffer is made. For example, if you make 0.2 M 100 ml buffer and you need 20 ml of 25 % glutaraldehyde and 80 ml of 0.2 M phosphate buffer for the preparation of 5 %. In 80 ml of 0.2 M phosphate buffer, add all the components such as monobasic and dibasic phosphate compound required for 100 ml. Finally 5% glutaraldehyde solution is prepared.
- Badges
- Science method