Cytokine immunofluorescence for tissue slices?

Hello, I am planning to do a cytokine immunoflourescence in spleen tissue slices. In bibliography most methods do not permeabilize cells, thereby detecting extracellular protein (associated to...

05 June 2018 9,441 3 View

BCR blocking antibody?

Hello everyone, Is there known antibody to block BCR function? Or any knock out mouse strain deficient in BCR signalling? Thanks in advance, Martin

01 February 2018 8,770 3 View

POVA(323-339) induced cytokine profile in DO11.10 mice?

06 July 2017 6,898 1 View

Enzymatic reaction scale-down?

Hi, I'm currently working with an enzymatic reaction. The reagents are quite expensive, so I wonder if I can scale down the reaction volume from 100ul to 25ul. Is this possible or there is any...

05 June 2017 3,642 6 View

Mucins and negative-charged proteins staining?

02 March 2017 3,914 3 View

Paraformaldehyde fixation conditions for intracellular staining?

Hello, I've been comparing intracellular staining protocols and noticed that there are several alternatives for fixation with paraformaldehyde, varying time and concentration. Also, I read that...

11 December 2016 586 5 View

IL-4 staining problem in Th2 polarized cells?

Hi, I'm having trouble trying to stain mouse IL-4 from Th2 cells polarized in vitro.I've been purifying CD4+ cells from BABL/c spleens with magnetic beads and culturing them in CD3/CD28 pre-coated...

10 November 2016 8,558 8 View

Avidin-Agarose resin high background?

Hello, I've been having trouble with biotynilated protein purification using Avidin-Agarose resin. The idea is to use the purified biotynilated molecules as samples for MALDI-TOF-MS.To check the...

06 July 2016 2,861 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View