I can't find anywhere what is the T helper profile induced by pOVA(323-339) in DO11.10 splenocytes. I was wondering what is the "starting point" of the cytokine profile using this model to study antigen-specific responses.
Try adoptive transfer DO11.10 T cells into Rag-/- mice and treat the mice with OVA peptides. Then set up different time points to study the cytokine profile of the ag-specific responses. Hope that helps.