I recently work on a microfluidics device, unlike the glass slide which is easy to perform the washing step after DNA hybridization (by serial washing buffers). I tried to do the same thing on the channels (by pipetting the washing buffers into the channel). However, after fluorescence scanning of the resulting hybridization, it showed that fluorescence stains remained along the channel (Which means ineffective washing step).
Do you have any other method for washing microfluidic channels, could you please share your experience ~
Thank you very much ~~
Microchannels are notoriously difficult to clean, especially when large sticky biomolecules are used. If the channels are not blocked, rinsing them for several minutes with a surfactant solution usually does the trick. I used to simply leave my chips attached to a syringe pump for half an hour or so, running SDS through the whole time; then a shorter ethanol rinse. Another commonly used solution is submerging the chips in an ultrasound bath. Some tip for this method can be found here:
http://blogs.rsc.org/chipsandtips/2016/02/08/efficient-cleaning-of-a-microfluidic-chip/
Thanks Eric for that interesting link - alternatively you can also have a look at:
Biotechniques. 2014 Nov 1;57(5):267-71. doi: 10.2144/000114232
Thank you @Eric and @Victor for your prompt reply. I will try with your advice and get back to this topic soon for updates ~
I have read the references and they are about cleaning and reusing the microfluidics chip, I just want to ask about the washing between steps such as: After surface probe bonding, blocking, and hybridization. Did you do the same way with that of the glass slide?
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